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| RIDER | NAT | TEAM | TIME | |
| 1 | Pol Espargaro | SPA | Repsol Honda (RC213V) | 1m 31.06s |
| 2 | Fabio Quartararo | FRA | Monster Yamaha (YZR-M1) | +0.215s |
| 3 | Aleix Espargaro | SPA | Aprilia Racing (RS-GP) | +0.325s |
| 4 | Francesco Bagnaia | ITA | Ducati Lenovo (GP22) | +0.376s |
| 5 | Alex Rins | SPA | Suzuki Ecstar (GSX-RR) | +0.417s |
| 6 | Maverick Viñales | SPA | Aprilia Racing (RS-GP) | +0.418s |
| 7 | Johann Zarco | FRA | Pramac Ducati (GP22) | +0.428s |
| 8 | Alex Marquez | SPA | LCR Honda (RC213V) | +0.543s |
| 9 | Luca Marini | ITA | Mooney VR46 Ducati (GP22) | +0.605s |
| 10 | Brad Binder | RSA | Red Bull KTM (RC16) | +0.682s |
| 11 | Takaaki Nakagami | JPN | LCR Honda (RC213V) | +0.758s |
| 12 | Jack Miller | AUS | Ducati Lenovo (GP22) | +0.810s |
| 13 | Andrea Dovizioso | ITA | WithU Yamaha RNF (YZR-M1) | +0.830s |
| 14 | Marco Bezzecchi | ITA | Mooney VR46 Ducati (GP21)* | +0.841s |
| 15 | Fabio Di Giannantonio | ITA | Gresini Ducati (GP21)* | +0.855s |
| 16 | Marc Marquez | SPA | Repsol Honda (RC213V) | +0.892s |
| 17 | Enea Bastianini | ITA | Gresini Ducati (GP21) | +0.950s |
| 18 | Miguel Oliveira | POR | Red Bull KTM (RC16) | +1.056s |
| 19 | Franco Morbidelli | ITA | Monster Yamaha (YZR-M1) | +1.109s |
| 20 | Jorge Martin | SPA | Pramac Ducati (GP22) | +1.484s |
| 21 | Remy Gardner | AUS | KTM Tech3 (RC16)* | +1.800s |
| 22 | Darryn Binder | RSA | WithU Yamaha RNF (YZR-M1)* | +1.989s |
| 23 | Raul Fernandez | SPA | KTM Tech3 (RC16)* | +3.836s |
*Rookie
Fastest Day 2 Time:
Luca Marini ITA Ducati 1m 31.289s
Fastest Day 1 Time:
Pol Espargaro SPA Honda 1m 32.466s
2021 Mandalika WorldSBK lap records:
Best lap:
Toprak Razgatlioglu TUR Yamaha 1m 32.877s
Fastest race lap:
Toprak Razgatlioglu TUR Yamaha 1m 34.288s
